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Thiobacillus thioparus Agar

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar, purified.............................................................................. 12.0g

Na2S2O3·5H2O ........................................................................... 10.0g

K2HPO4......................................................................................... 4.0g

KH2PO4......................................................................................... 4.0g

MgSO4·7H2O ................................................................................ 0.1g

(NH4)2SO4..................................................................................... 0.1g

CaCl2............................................................................................. 0.1g

FeCl3·6H2O ................................................................................. 0.02g

MnSO4·H2O ................................................................................ 0.02g

pH 6.6 ± 0.2 at 25°C

Preparation of Medium: Add components, except agar, to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad

just pH to 6.6. Add agar. Gently heat and bring to boiling. Distribute

into tubes or flasks. Autoclave for 20 min at 10 psi pressure–115°C.

Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Thiobacillus thioparus.