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Thiobacillus thyasiris Broth

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Solution B ..............................................................................900.0mL

Solution A..............................................................................100.0mL

Vitamin solution.......................................................................10.0mL

pH 7.3–7.6 at 25°C

Solution A:

Composition per 100.0mL:

Na2HPO42H2O.............................................................................. 7.9g

KH2PO4......................................................................................... 1.5g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.4.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper

ature.

Solution B:

Composition per 900.0mL:

NaCl............................................................................................ 25.1g

Na2S2O3 ........................................................................................ 5.0g

NH4Cl ........................................................................................... 0.4g

MgSO4·7H2O................................................................................ 0.1g

Phenol Red................................................................................. 3.0mg

Trace elements solution ...........................................................10.0mL

Trace Elements Solution:

Composition per liter:

Disodium EDTA ......................................................................... 50.0g

NaOH......................................................................................... 11.0g

ZnSO4·7H2O ............................................................................... 11.0g

CaCl2·2H2O ................................................................................ 7.34g

FeSO4·7H2O.................................................................................. 5.0g

MnCl2·4H2O ................................................................................. 2.5g

CoCl2·6H2O .................................................................................. 0.5g

(NH4)6MoO24·4H2O...................................................................... 0.5g

CuSO4·5H2O ................................................................................. 0.2g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Adjust pH to 6.0.

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to room temperature.

Vitamin Solution:

Composition per liter:

Calcium DL-pantothenate.........................................................20.0mg

Nicotinic acid...........................................................................20.0mg

Pyridoxine·HCl ........................................................................20.0mg

Riboflavin ................................................................................20.0mg

p-Aminobenzoic acid...............................................................10.0mg

Thiamine·HCl ..........................................................................10.0mg

Biotin .........................................................................................1.0mg

Vitamin B12 ................................................................................1.0mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH

to 7.0. Filter sterilize.

Preparation of Medium: Aseptically combine 100.0mL of sterile

solution A with 900.0mL of sterile solution B and 10.0mL of sterile vi

tamin solution. Mix thoroughly. Aseptically distribute into sterile tubes

or flasks.

Use: For the cultivation and maintenance of Thiobacillus thyasiris.

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Thiobacillus thyasiris Broth