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Thiobacillus thyasiris/Thiobacillus halophilus Medium (DSMZ Medium 484)

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

Thiobacillus thyasiris/Thiobacillus halophilus Medium

(DSMZ Medium 484)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Solution B ..............................................................................900.0mL

Solution A ..............................................................................100.0mL

Vitamin solution.......................................................................10.0mL

pH 7.3–7.6 at 25°C

Solution A:

Composition per 100.0mL:

Na2HPO4·2H2O............................................................................. 7.9g

KH2PO4......................................................................................... 1.5g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.6.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Solution B:

Composition per 900.0mL:

NaCl ............................................................................................ 25.1g

Na2S2O3 ........................................................................................ 5.0g

NH4Cl ........................................................................................... 0.4g

MgSO4·7H2O ................................................................................ 0.1g

Phenol Red.................................................................................3.0mg

Trace elements solution ...........................................................10.0mL

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to room temperature.

Trace Elements Solution:

Composition per liter:

Na2-EDTA................................................................................... 50.0g

NaOH .......................................................................................... 11.0g

ZnSO4·7H2O ............................................................................... 11.0g

CaCl2·2H2O ................................................................................ 7.34g

FeSO4·7H2O.................................................................................. 5.0g

MnCl2·4H2O ................................................................................. 2.5g

CoCl2·6H2O .................................................................................. 0.5g

(NH4)6Mo7O24·4H2O .................................................................... 0.5g

CuSO4·5H2O................................................................................. 0.2g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Vitamin Solution:

Composition per liter:

Riboflavin ................................................................................ 20.0mg

Ca-pantothenate ....................................................................... 20.0mg

Nicotinic acid........................................................................... 20.0mg

Pyridoxine-HCl........................................................................ 20.0mg

p-Aminobenzoic acid............................................................... 10.0mg

Thiamine-HCl·2H2O................................................................ 10.0mg

Biotin ......................................................................................... 1.0mg

Vitamin B12 ................................................................................ 1.0mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Aseptically combine 100.0mL solution

A, 900.0mL solution B, and 10.0mL vitamin solution. Mix thoroughly.

Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiobacillus thyasiris,

Thiobacillus halophilus, Thiomicrospira thyasirae, and Halothiobacil

lus halophilus DSM6132.

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Thiobacillus thyasiris/Thiobacillus halophilus Medium (DSMZ Medium 484)