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培养基配方

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Thiocapsa halophila Medium

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1061.0mL:

NaCl............................................................................................ 70.0g

MgCl2·6H2O ................................................................................. 4.5g

MgSO4·7H2O................................................................................ 3.0g

NH4Cl ........................................................................................... 0.5g

KH2PO4......................................................................................... 0.3g

CaCl2·2H2O ................................................................................ 0.05g

NaHCO3 solution.....................................................................40.0mL

Na2S·9H2O solution.................................................................10.0mL

Na2S2O3 solution .....................................................................10.0mL

Vitamin B12 solution ..................................................................1.0mL

Trace elements solution SL-12B................................................1.0mL

pH 7.2 ± 0.2 at 25°C

NaHCO3 Solution:

Composition per 100.0mL:

NaHCO3........................................................................................ 5.0g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Na2S·9H2O Solution:

Composition per 10.0mL:

Na2S·9H2O.................................................................................... 0.6g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Na2S2O3 Solution :

Composition per 10.0mL:

Na2S2O3 ·5H2O.............................................................................. 0.5g

Preparation of Na2S2O3 Solution: Add Na2S2O3·5H2O to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Vitamin B12 Solution:

Composition per 10.0mL:

Vitamin B12 ................................................................................0.2mg

Preparation of Vitamin B12 Solution: Add vitamin B12 to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Trace Elements Solution SL-12B:

Composition per liter:

MnCl2·2H2O................................................................................ 50.0g

Disodium EDTA ........................................................................... 3.0g

FeSO4·7H2O................................................................................. 1.1g

H3BO3 ........................................................................................... 0.3g

CoCl2·6H2O ................................................................................ 0.19g

ZnCl2........................................................................................42.0mg

NiCl2·6H2O ..............................................................................24.0mg

Na2MoO4·2H2O ...................................................................... 18.0mg

CuCl2·2H2O ...............................................................................2.0mg

Preparation of Trace Elements Solution SL-12B: Add compo

nents to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly.

Preparation of Medium: Add components, except NaHCO3 solu

tion, Na2S·9H2O solution, Na2S2O3 solution, and vitamin B12 solution,

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper

ature while sparging with 90% N2 + 10% CO2. Aseptically and anaer

obically add 40.0mL of sterile NaHCO3 solution, 10.0mL of sterile

Na2S·9H2O solution, 10.0mL of sterile Na2S2O3 solution, and 1.0mL

of sterile vitamin B12 solution. Mix thoroughly. Aseptically and anaer

obically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiocapsa halophila.

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Thiocapsa halophila Medium