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Thiocyanate Agar

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

Thiocyanate Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A ..............................................................................800.0mL

Solution B ..............................................................................100.0mL

Solution C ..............................................................................100.0mL

Solution A:

Composition per 800.0mL:

Agar, noble.................................................................................. 30.0g

K2HPO4......................................................................................... 1.0g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O ................................................................................ 0.2g

CaCl2........................................................................................20.0mg

FeCl3·6H2O (60%) .....................................................................0.1mL

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

50°–55°C.

Solution B:

Composition per 100.0mL:

KCNS............................................................................................ 3.6g

Preparation of Solution B: Add KCNS to distilled/deionized wa

ter and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°–55°C.

Solution C:

Composition per 100.0mL:

Disodium succinate....................................................................... 1.5g

Preparation of Solution C: Add disodium succinate to distilled/

deionized water and bring volume to 100.0mL. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Preparation of Medium: Aseptically combine 800.0mL of solu

tion A with 100.0mL of solution B and 100.0mL of solution C. Mix

thoroughly. Pour into sterile Petri dishes or aseptically distribute into

sterile tubes.

Use: For the cultivation and maintenance of a variety of microorgan

isms that can utilize thiocyanante as sole source of nitrogen and sulfur.