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Thiocyanate Utilization Medium

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1225.0mL:

Basal solution................................................................................1.0L

Solution C ..............................................................................200.0mL

Solution B ................................................................................20.0mL

Solution A..................................................................................5.0mL

Basal Solution:

Composition per liter:

Na2HPO4....................................................................................... 4.8g

KH2PO4......................................................................................... 4.4g

MgSO4·7H2O................................................................................ 0.5g

Preparation of Basal Solution: Add components to distilled/de

ionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution A:

Composition per 100.0mL:

FeCl3·6H2O................................................................................... 1.0g

CaCl2............................................................................................. 0.1g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Solution B:

Composition per 100.0mL:

D-Glucose.................................................................................... 10.0g

Preparation of Solution B: Add glucose to distilled/deionized wa

ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Solution C:

Composition per 200.0mL:

NaSCN.......................................................................................... 1.0g

Preparation of Solution C: Add NaSCN to distilled/deionized wa

ter and bring volume to 200.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 1.0L of cooled, sterile basal solution,

aseptically add 5.0mL of sterile solution A, 20.0mL of sterile solution

B, and 200.0mL of sterile solution C. Mix thoroughly. Aseptically dis

tribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of a variety of microorgan

isms that can utilize thiocyanate as sole source of nitrogen and sulfur.