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Thiogel® Medium

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

Thiogel® Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Gelatin......................................................................................... 50.0g

Pancreatic digest of casein.......................................................... 17.0g

Glucose ......................................................................................... 6.0g

Papaic digest of soybean meal...................................................... 3.0g

NaCl.............................................................................................. 2.5g

Sodium thioglycolate.................................................................... 0.5g

Agar .............................................................................................. 0.7g

Na2SO3.......................................................................................... 0.1g

L-Cystine ..................................................................................... 0.25g

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water preheated to 50°C and bring volume to 1.0L. Mix thoroughly. Let

stand for 5 min. Gently heat while stirring and bring to boiling. Distrib

ute into tubes, filling them half full. Autoclave for 15 min at 13 psi

pressure–118°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the differentiation of microorganisms based on their ability

to liquefy gelatin.