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Thioglycolate Bile Broth

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1050.0mL:

Pancreatic digest of casein.......................................................... 15.0g

Glucose ......................................................................................... 5.5g

Yeast extract.................................................................................. 5.0g

NaCl.............................................................................................. 2.5g

Agar ............................................................................................ 0.75g

L-Cystine ....................................................................................... 0.5g

Sodium thioglycolate.................................................................... 0.5g

Bile solution.............................................................................50.0mL

pH 7.1 ± 0.2 at 25°C

Bile Solution:

Composition per 100.0mL:

Oxgall ......................................................................................... 40.0g

Sodium deoxycholate.................................................................... 2.0g

Preparation of Bile Solution: Add components to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, except bile solution, to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Gently heat and bring to boiling. Distribute into tubes in 10.0mL vol-

umes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–

50°C. Aseptically add 0.5mL of sterile bile solution to each tube. Mix

thoroughly.

Use: For the cultivation of Bacteroides fragilis and Clostridium per

fringens from clinical specimens.