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Thioglycolate Gelatin Medium

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Gelatin......................................................................................... 50.0g

Pancreatic digest of casein.......................................................... 15.0g

Yeast extract.................................................................................. 5.0g

NaCl .............................................................................................. 2.5g

Glucose ......................................................................................... 2.0g

Agar ............................................................................................ 0.75g

L-Cystine ..................................................................................... 0.25g

Na2SO3 .......................................................................................... 0.1g

Thioglycollic acid ......................................................................0.3mL

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to 50°C. Let stand 5 min. Gently heat and bring to boiling. Distribute

into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the determination of gelatin liquefaction by aerobes, micro

aerophiles, and anaerobes without special incubation.