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Thioglycolate HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Plant hydrolysate......................................................................... 15.0g

Glucose ......................................................................................... 5.5g

Yeast extract.................................................................................. 5.0g

NaCl .............................................................................................. 2.5g

L-Cystine....................................................................................... 0.5g

Na-thioglycolate............................................................................ 0.5g

Resazurin ...................................................................................1.0mg

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Prepare

freshly or boil and cool the medium just before use. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the cultivation of facultative and anaerobic organisms. For

the performance of sterility tests of turbid or viscous specimens.