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Thioglycolate Medium (DSMZ Medium 530)

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

Thioglycolate Medium

(DSMZ Medium 530)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Peptone from casein...................................................................... 5.0g

Meat extract .................................................................................. 3.0g

Na-thioglycolate solution.......................................................100.0mL

pH 5.5 ± 0.2 at 25°C

Thioglycolate Solution :

Composition per 100.0mL:

Na-thioglycolate ........................................................................... 0.5g

Preparation of Thioglycolate Solution: Add thioglycolate to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Filter sterilize. Warm to 50°C.

Preparation of Medium: Add components, except thioglycolate

solution, to distilled/deionized water and bring volume to 900.0mL.

Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 100.0mL

warm thioglycolate solution. Adjust pH to 5.5. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of various anaerobic bacteria.