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Thioglycolate Medium with 20% Bile (THIO + Bile Medium)

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

Thioglycolate Medium with 20% Bile

(THIO + Bile Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Oxgall ......................................................................................... 20.0g

Thioglycolate medium without indicator......................................1.0L

Hemin solution...........................................................................0.5mL

Vitamin K1 solution ...................................................................0.1mL

pH 7.0 ± 0.2 at 25°C

Thioglycolate Medium without Indicator:

Composition per liter:

Pancreatic digest of casein.......................................................... 17.0g

Glucose ......................................................................................... 6.0g

Papaic digest of soybean meal ...................................................... 3.0g

NaCl .............................................................................................. 2.5g

Agar .............................................................................................. 0.7g

Sodium thioglycolate .................................................................... 0.5g

L-Cystine ..................................................................................... 0.25g

Na2SO3.......................................................................................... 0.1g

Preparation of Thioglycolate Medium Without Indicator:

Add components to distilled/deionized water and bring volume to 1.0L.

Mix thoroughly.

Vitamin K1 Solution:

Composition per 100.0mL:

Vitamin K1 .................................................................................... 1.0g

Preparation of Vitamin K1 Solution: Add vitamin K1 to 99.0mL

of absolute ethanol. Mix thoroughly.

Hemin Solution:

Composition per 100.0mL:

Hemin............................................................................................ 1.0g

NaOH (1N solution).................................................................20.0mL

Preparation of Hemin Solution: Add hemin to 20.0mL of 1N

NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis

tilled/deionized water.

Preparation of Medium: Add 0.5mL of hemin solution, 0.1mL of

vitamin K1 solution, and oxgall to 1.0L of thioglycolate medium with

out indicator. Mix thoroughly. Distribute into screw-capped tubes or

flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool tubes or

flasks under 85% N2 + 10% H2 + 5% CO2. Tighten caps.

Use: For the isolation, cultivation, and identification of a variety of obli

gate anaerobic bile-tolerant bacteria.