培养基配方

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Thioglycolate Medium, Brewer Modified

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 17.5g

Glucose ....................................................................................... 10.0g

NaCl.............................................................................................. 5.0g

Papaic digest of soybean meal...................................................... 2.5g

K2HPO4......................................................................................... 2.0g

Sodium thioglycolate.................................................................... 1.0g

Agar .............................................................................................. 0.5g

Methylene Blue......................................................................... 0.002g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Distribute into tubes or flasks, filling them

half full. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of obligate anaerobes, microaerophiles, and

facultative organisms.