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Thioglycolate Medium, Enriched (THIO Medium) (Thioglycolate Medium with Vitamin K1 and Hemin)

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

Thioglycolate Medium, Enriched

(THIO Medium)

(Thioglycolate Medium with Vitamin K1 and Hemin)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Thioglycolate medium without indicator......................................1.0L

Hemin solution...........................................................................0.5mL

Vitamin K1 solution ...................................................................0.1mL

pH 7.0 ± 0.2 at 25°C

Thioglycolate Medium without Indicator:

Composition per liter:

Pancreatic digest of casein.......................................................... 17.0g

Glucose ......................................................................................... 6.0g

Papaic digest of soybean meal...................................................... 3.0g

NaCl.............................................................................................. 2.5g

Agar .............................................................................................. 0.7g

Sodium thioglycolate.................................................................... 0.5g

L-Cystine ..................................................................................... 0.25g

Na2SO3.......................................................................................... 0.1g

Source: Thioglycolate medium without indicator is available as a pre

mixed powder from Oxoid Unipath and BD Diagnostic Systems.

Preparation of Thioglycolate Medium without Indicator:

Add components to distilled/deionized water and bring volume to

1.0L. Mix thoroughly.

Vitamin K1 Solution:

Composition per 100.0mL:

Vitamin K1 .................................................................................... 1.0g

Preparation of Vitamin K1 Solution: Add vitamin K1 to 99.0mL

of absolute ethanol. Mix thoroughly.

Hemin Solution:

Composition per 100.0mL:

Hemin ........................................................................................... 1.0g

NaOH (1N solution).................................................................20.0mL

Preparation of Hemin Solution: Add hemin to 20.0mL of 1N

NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis

tilled/deionized water.

Preparation of Medium: Add 0.5mL of hemin solution and 0.1mL

of vitamin K1 solution to 1.0L of thioglycolate medium without indi-

cator. Mix thoroughly. Distribute into screw-capped tubes or flasks.

Autoclave for 15 min at 15 psi pressure–121°C. Cool tubes or flasks

under 85% N2 + 10% H2 + 5% CO2. Tighten caps.

Use: For the isolation, cultivation, and identification of a wide variety

of obligate anaerobic bacteria.

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Thioglycolate Medium, Enriched (THIO Medium) (Thioglycolate Medium with Vitamin K1 and Hemin)