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Thioglycolate Medium without Indicator-135C

[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 17.0g

Glucose ......................................................................................... 6.0g

Papaic digest of soybean meal ...................................................... 3.0g

NaCl .............................................................................................. 2.5g

Agar .............................................................................................. 0.7g

Sodium thioglycolate .................................................................... 0.5g

Na2SO3.......................................................................................... 0.1g

L-Cystine ..................................................................................... 0.25g

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Distribute into tubes or flasks, filling them

half full. For maintenance of cultures, a small quantity of CaCO3 may

be added to tubes before adding medium. Autoclave for 15 min at 13

psi pressure–118°C. Prepare freshly or boil and cool the medium just

before use. Store prepared medium at 2°–8°C in the dark.

Use: For the isolation and cultivation of a wide variety of microorgan

isms, particularly obligate anaerobes, from clinical specimens and

other materials.