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Thioglycolate Medium without Indicator with Hemin (Thioglycolate Medium, Supplemented)

[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]

Thioglycolate Medium without Indicator with Hemin

(Thioglycolate Medium, Supplemented)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 17.0g

CaCO3, chips or powder ............................................................. 10.0g

Glucose ......................................................................................... 6.0g

Papaic digest of soybean meal ...................................................... 3.0g

NaCl .............................................................................................. 2.5g

Agar .............................................................................................. 0.7g

Sodium thioglycolate .................................................................... 0.5g

L-Cystine ..................................................................................... 0.25g

Na2SO3.......................................................................................... 0.1g

Hemin ........................................................................................ 5.0mg

Na2CO3 solution......................................................................10.0mL

Vitamin K1 solution .................................................................10.0mL

pH 7.2 ± 0.2 at 25°C

Na2CO3 Solution:

Composition per 10.0mL:

Na2CO3 ......................................................................................... 1.0g

Preparation of Na2CO3 Solution: Add Na2CO3 to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Vitamin K1 Solution:

Composition per 100.0mL:

Vitamin K1 .................................................................................... 1.0g

Ethanol, absolute......................................................................99.0mL

Preparation of Vitamin K1 Solution: Add vitamin K1 to 99.0mL

of absolute ethanol. Mix thoroughly.

Preparation of Medium: Add components, except CaCO3, Na2CO3

solution, and vitamin K1 solution, to distilled/deionized water and

bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to

boiling. Add 0.1g of CaCO3 chips or powder to each of 100 test tubes.

Distribute broth into the same tubes in 10.0mL volumes. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add

0.1mL of sterile Na2CO3 solution and 0.1mL of sterile vitamin K1 so

lution to each tube. Mix thoroughly.

Use: For the cultivation of a wide variety of obligate anaerobes.