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Thiomicrospira denitrificans Broth

[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Solution A ..............................................................................940.0mL

Solution B ................................................................................40.0mL

Solution C ................................................................................20.0mL

Solution D ..................................................................................1.0mL

pH 7.0 ± 0.2 at 25°C

Solution A:

Composition per 940.0mL:

KH2PO4......................................................................................... 2.0g

KNO3 ............................................................................................ 2.0g

NH4Cl ........................................................................................... 1.0g

MgSO4·7H2O................................................................................ 0.8g

Trace elements solution SL-4 ....................................................2.0mL

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 940.0mL. Mix thoroughly. Adjust pH to 7.0

with NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C.

Solution B:

Composition per 40.0mL:

Na2S2O3·5H2O .............................................................................. 5.0g

Preparation of Solution B: Add Na2S2O3·5H2O to distilled/deion

ized water and bring volume to 40.0mL. Mix thoroughly. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution C:

Composition per 20.0mL:

NaHCO3........................................................................................ 1.0g

Preparation of Solution C: Add NaHCO3 to distilled/deionized

water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.

Solution D:

Composition per liter:

FeSO4·7H2O............................................................................... 2.0mg

H2SO4 (0.1N solution) ...............................................................1.0mL

Preparation of Solution D: Add FeSO4·7H2O to 1.0mL of 0.1N

H2SO4 solution. Mix thoroughly. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C.

Trace Elements Solution SL-4:

Composition per liter:

EDTA............................................................................................ 0.5g

FeSO4·7H2O.................................................................................. 0.2g

Trace elements solution SL-6 ................................................100.0mL

Preparation of Trace Elements Solution SL-4: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Trace Elements Solution SL-6:

Composition per liter:

H3BO3 ........................................................................................... 0.3g

CoCl2·6H2O .................................................................................. 0.2g

ZnSO4·7H2O ................................................................................. 0.1g

MnCl2·4H2O ............................................................................... 0.03g

Na2MoO4·H2O ............................................................................ 0.03g

NiCl2·6H2O................................................................................. 0.02g

CuCl2·2H2O................................................................................. 0.01g

Preparation of Trace Elements Solution SL-6: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad

just pH to 3.4.

Preparation of Medium: Aseptically add 40.0mL of sterile solution

B, 20.0mL of sterile solution C, and 1.0mL of sterile solution D to

940.0mL of sterile solution A. Mix thoroughly. Aseptically and anaero

bically distribute into sterile tubes under 100% N2.

Use: For the cultivation and maintenance of Thiomicrospira denitrifi

cans.

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Thiomicrospira denitrificans Broth