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Thiomicrospira denitrificans Medium

[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Part I.......................................................................................500.0mL

Part II .....................................................................................500.0mL

pH 7.0–8.0 at 25°C

Part I:

Composition per liter:

NaCl ............................................................................................ 20.0g

KNO3 ............................................................................................ 4.0g

(NH4)2SO4..................................................................................... 2.0g

MgSO4·7H2O ................................................................................ 1.5g

K2HPO4......................................................................................... 0.6g

KH2PO4......................................................................................... 0.4g

FeSO4 solution ...........................................................................2.0mL

Trace metals solution .................................................................2.0mL

HCl, concentrated ......................................................................1.0mL

Preparation of Part I: Add components to distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 25°C.

FeSO4 Solution:

Composition per 100.0mL:

FeSO4·7H2O.................................................................................. 0.5g

HCl (1N solution)...................................................................100.0mL

Preparation of FeSO4 Solution: Combine the FeSO4·7H2O and

100.0mL of HCl solution. Mix thoroughly.

Trace Metals Solution:

Composition per liter:

Disodium EDTA ......................................................................... 50.0g

NaOH .......................................................................................... 11.0g

CaCl2·2H2O................................................................................. 7.34g

MnCl2·2H2O.................................................................................. 2.5g

ZnSO4·7H2O ................................................................................. 2.2g

CoCl2·6H2O .................................................................................. 0.5g

(NH4)6Mo7O24·4H2O .................................................................... 0.5g

CuSO4·5H2O ................................................................................. 0.2g

Preparation of Trace Metals Solution: Add EDTA to distilled/

deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust

pH to 6.0 with NaOH. Add remaining components, one by one. Main

tain the pH at 6.0. After dissolution of all the salts, adjust the pH to 4.0

with HCl. Store at 4°C.

Part II:

Composition per liter:

Na2S2O3·5H2O ............................................................................ 10.0g

NaHCO3 ........................................................................................ 3.0g

NaOH .......................................................................................... 0.05g

Preparation of Part II: Add components to distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 25°C.

Preparation of Medium: Aseptically combine 500.0mL of sterile

part I and 500.0mL of sterile part II. Mix thoroughly. Aseptically dis

tribute into sterile tubes or flasks.

Use: For the isolation and cultivation of Thiomicrospira denitrificans.

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Thiomicrospira denitrificans Medium