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培养基配方
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Thiomicrospira Medium (ATCC Medium 1422)
[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]
Thiomicrospira Medium
(ATCC Medium 1422)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
NaCl............................................................................................ 25.1g
Tris·HCl ...................................................................................... 3.07g
Na2S2O3·5H2O ............................................................................ 2.48g
MgSO4·7H2O................................................................................ 1.5g
(NH4)2SO4 .................................................................................... 1.0g
KH2PO4....................................................................................... 0.42g
CaCl2·2H2O ................................................................................ 0.29g
NaHCO3........................................................................................ 0.2g
Phenol Red (0.5% solution).......................................................1.0mL
Vishniac and Santer trace metals ...............................................0.2mL
pH 7.5 ± 0.2 at 25°C
Vishniac and Santer Trace Metals:
Composition per liter:
Ethylenediamine tetraacetic acid (EDTA) .................................. 50.0g
ZnSO4·7H2O ............................................................................... 22.0g
CaCl2........................................................................................... 5.54g
MnCl2·4H2O ............................................................................... 5.06g
FeSO4·7H2O................................................................................ 4.99g
CoCl2·6H2O ................................................................................ 1.61g
CuSO4·5H2O............................................................................... 1.57g
(NH4)6Mo7O24·4H2O .................................................................... 1.1g
Preparation of Vishniac and Santer Trace Metals: Add com
ponents to distilled/deionized water and bring volume to 1.0L. Adjust
pH to 6.0 with KOH. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5. Fil
ter sterilize. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Thiomicrospira species.