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Thiomicrospira Medium (ATCC Medium 1422)

[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]

Thiomicrospira Medium

(ATCC Medium 1422)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................ 25.1g

Tris·HCl ...................................................................................... 3.07g

Na2S2O3·5H2O ............................................................................ 2.48g

MgSO4·7H2O................................................................................ 1.5g

(NH4)2SO4 .................................................................................... 1.0g

KH2PO4....................................................................................... 0.42g

CaCl2·2H2O ................................................................................ 0.29g

NaHCO3........................................................................................ 0.2g

Phenol Red (0.5% solution).......................................................1.0mL

Vishniac and Santer trace metals ...............................................0.2mL

pH 7.5 ± 0.2 at 25°C

Vishniac and Santer Trace Metals:

Composition per liter:

Ethylenediamine tetraacetic acid (EDTA) .................................. 50.0g

ZnSO4·7H2O ............................................................................... 22.0g

CaCl2........................................................................................... 5.54g

MnCl2·4H2O ............................................................................... 5.06g

FeSO4·7H2O................................................................................ 4.99g

CoCl2·6H2O ................................................................................ 1.61g

CuSO4·5H2O............................................................................... 1.57g

(NH4)6Mo7O24·4H2O .................................................................... 1.1g

Preparation of Vishniac and Santer Trace Metals: Add com

ponents to distilled/deionized water and bring volume to 1.0L. Adjust

pH to 6.0 with KOH. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5. Fil

ter sterilize. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiomicrospira species.