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Thiomicrospira pelophila Medium

[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ............................................................................................ 25.0g

Agar ............................................................................................ 10.0g

Na2S2O3·5H2O ....................................................................... 5.0–8.0g

MgSO4·7H2O ................................................................................ 1.5g

(NH4)2SO4..................................................................................... 1.0g

K2HPO4......................................................................................... 0.5g

CaCl2............................................................................................. 0.3g

Vitamin B12 ..............................................................................0.15mg

Trace metals solution .................................................................0.2mL

Trace Metals Solution:

Composition per liter:

Disodium EDTA ......................................................................... 50.0g

NaOH .......................................................................................... 11.0g

CaCl2·2H2O................................................................................. 7.34g

FeSO4·7H2O.................................................................................. 5.0g

MnCl2·2H2O.................................................................................. 2.5g

ZnSO4·7H2O ................................................................................. 2.2g

CoCl2·6H2O .................................................................................. 0.5g

(NH4)6Mo7O24·4H2O .................................................................... 0.5g

CuSO4·5H2O ................................................................................. 0.2g

Preparation of Trace Metals Solution: Add EDTA to distilled/

deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust

pH to 6.0 with NaOH. Add remaining components, one by one. Main

tain the pH at 6.0. After dissolution of all the salts, adjust the pH to 4.0

with HCl. Store at 4°C.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Thiomicrospira pelophila.