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培养基配方

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Thiomicrospira pelophila Medium

[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1000.2mL:

NaCl ............................................................................................ 25.0g

MgSO4·7H2O ............................................................................... 1.5g

(NH4)2SO4..................................................................................... 1.0g

CaCl2·2H2O................................................................................. 0.42g

Bromthymol Blue ......................................................................4.0mg

K2HPO4 solution ....................................................................100.0mL

Na2S2O3·5H2O solution .........................................................100.0mL

Vitamin B12 solution ................................................................10.0mL

Trace elements solution .............................................................0.2mL

Na2CO3 solution ..................................................................... variable

pH 7.2 ± 0.2 at 25°C

K2HPO4 Solution:

Composition per 100.0mL:

K2HPO4......................................................................................... 0.5g

Preparation of K2HPO4 Solution: Add K2HPO4 to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C.

Na2S2O3 Solution:

Composition per 10.0mL:

Na2S2O3 ·5H2O.............................................................................. 5.0g

Preparation of Na2S2O3 Solution: Add Na2S2O3·5H2O to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C.

Vitamin B12 Solution:

Composition per 10.0mL:

Vitamin B12 .............................................................................. 15.0mg

Preparation of Vitamin B12 Solution: Add vitamin B12 to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Trace Elements Solution:

Composition per liter:

Disodium EDTA ......................................................................... 50.0g

ZnSO4·7H2O .............................................................................. 22.0g

CaCl2·2H2O ................................................................................ 5.54g

MnCl2·4H2O ............................................................................... 5.06g

FeSO4·7H2O.................................................................................. 5.0g

CoCl2·6H2O ................................................................................ 1.61g

CuSO4·5H2O............................................................................... 1.57g

(NH4)6Mo7O24·4H2O .................................................................... 1.1g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Na2CO3 Solution:

Composition per 100.0mL:

Na2CO3 ......................................................................................... 0.4g

Preparation of Na2CO3 Solution: Add Na2CO3 to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except K2HPO4 solu

tion, Na2S2O3·5H2O solution, vitamin B12 solution, and Na2CO3 solu

tion, to distilled/deionized water and bring volume to 790.0mL. Mix

thoroughly. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure–

121°C. Aseptically add 100.0mL of sterile K2HPO4 solution, 100.0mL

of sterile Na2S2O3·5H2O solution, and 10.0mL of sterile vitamin B12

solution. Mix thoroughly. Aseptically adjust pH to 7.2 with the appro

priate volume of sterile Na2CO3 solution. Aseptically distribute into

sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiomicrospira pelo

phila.

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Thiomicrospira pelophila Medium