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Thiophaeococcus mangrovi Medium (DSMZ Medium 1162)

[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]

Thiophaeococcus mangrovi Medium

(DSMZ Medium 1162)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ............................................................................................ 20.0g

Sodium pyruvate ........................................................................... 3.0g

MgSO4·7H2O ................................................................................ 2.0g

NH4Cl ........................................................................................ 0.64g

K2HPO4......................................................................................... 0.5g

Yeast extract ................................................................................. 0.4g

CaCl2·2H2O................................................................................. 0.15g

Bicarbonate solution ................................................................10.0mL

Sulfide solution ........................................................................10.0mL

Thiosulfate solution .................................................................10.0mL

Trace element solution SL-8......................................................1.0mL

Vitamin solution.........................................................................1.0mL

pH 7.5 ± 0.2 at 25°C

Sulfide Solution:

Composition per 10.0mL:

Na2S·9H2O.................................................................................... 1.0g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Sparge

with 100% N2. Filter sterilize.

Thiosulfate Solution:

Composition per 10.0mL:

Na2S2O3·5H2O .............................................................................. 1.5g

Preparation of Thiosulfate Solution: Add Na2S2O3·5H2O to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Sparge with 100% N2. Filter sterilize.

Bicarbonate Solution:

Composition per 10.0mL:

NaHCO3 ........................................................................................ 1.0g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge

with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Vitamin Solution:

Composition per 10.0mL:

Vitamin B12 ................................................................................2.0mg

Preparation of Vitamin Solution: Add Vitamin B12 to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Trace Elements Solution SL-8:

Composition per liter:

Disodium EDTA ........................................................................... 5.2g

FeCl2·4H2O ................................................................................... 1.5g

CoCl2·6H2O ................................................................................ 0.19g

MnCl2·4H2O ................................................................................. 0.1g

ZnCl2........................................................................................... 0.07g

H3BO3 ......................................................................................... 0.06g

NaMoO4·2H2O............................................................................ 0.04g

CuCl2·2H2O ................................................................................ 0.02g

NiCl2·6H20.................................................................................. 0.02g

Preparation of Trace Elements Solution SL-8: Add compo

nents to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly.

Preparation of Medium: Add components, except sulfide, bicar

bonate, thiosulfate, and vitamin solutions, to distilled/deionized water

and bring volume to 970.0mL. Mix thoroughly. Sparge with 80% N2 +

20% CO2 gas mixture. Dispense into culture vessels. Autoclave for 15

min at 15 psi pressure–121°C. Cool to room temperature. Aseptically

add sulfide, thiosulfate, bicarbonate, and vitamin solutions. Mix thor

oughly. Adjust pH to 7.5.

Use: For the cultivation of Thiophaeococcus mangrovi.

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Thiophaeococcus mangrovi Medium (DSMZ Medium 1162)