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Thiosphaera Agar

[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]

Thiosphaera Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Na2HPO4....................................................................................... 4.2g

KH2PO4......................................................................................... 1.5g

NH4Cl ........................................................................................... 0.3g

MgSO4·7H2O................................................................................ 0.1g

KNO3 ............................................................................................ 0.1g

Vishniac and Santer trace metals ...............................................2.0mL

pH 8.0–8.2 at 25°C

Vishniac and Santer Trace Metals:

Composition per liter:

Ethylenediamine tetraacetic acid (EDTA) .................................. 50.0g

ZnSO4·7H2O ............................................................................... 22.0g

CaCl2........................................................................................... 5.54g

MnCl2·4H2O ............................................................................... 5.06g

FeSO4·7H2O................................................................................ 4.99g

CoCl2·6H2O ................................................................................ 1.61g

CuSO4·5H2O............................................................................... 1.57g

(NH4)6Mo7O24·4H2O .................................................................... 1.1g

Preparation of Vishniac and Santer Trace Metals: Add com

ponents to distilled/deionized water and bring volume to 1.0L. Adjust

pH to 6.0 with KOH. Mix thoroughly.

Preparation of Medium: Add components, except agar, to dis

tilled/deionized water and bring volume to 500.0mL. Mix thoroughly.

Adjust pH to 8.0–8.2. Filter sterilize. Warm to 45°–50°C. Add agar to

distilled/deionized water and bring volume to 500.0mL. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically combine the two ster

ile solutions. Mix thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the cultivation and maintenance of Thiosphaera pantotro

pha.