培养基配方

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Thiosphaera pantotropha Medium

[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Agar ............................................................................................ 20.0g

Na2HPO4·2H2O............................................................................ .7.9g

KH2PO4......................................................................................... 1.5g

NH4Cl ........................................................................................... 0.3g

MgSO4·7H2O ................................................................................ 0.1g

Yeast extract solution...............................................................10.0mL

Trace elements solution SL-10 ..................................................1.0mL

pH 7.5 ± 0.2 at 25°C

Yeast Extract Solution:

Composition per 10.0mL:

Yeast extract.................................................................................. 1.0g

Preparation of Yeast Extract Solution: Add yeast extract to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C.

Trace Elements Solution SL-10:

Composition per liter:

FeCl2·4H2O ................................................................................... 1.5g

CoCl2·6H2O ...........................................................................190.0mg

MnCl2·4H2O...........................................................................100.0mg

ZnCl2........................................................................................70.0mg

Na2MoO4·2H2O .......................................................................36.0mg

NiCl2·6H2O ..............................................................................24.0mg

H3BO3 ........................................................................................6.0mg

CuCl2·2H2O ...............................................................................2.0mg

HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized

water and bring volume to 1.0L. Add remaining components. Mix thor

oughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–

121°C.

Preparation of Medium: Add components, except yeast extract so

lution, to distilled/deionized water and bring volume to 990.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 10.0mL of

sterile yeast extract solution. Mix thoroughly. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Paracoccus denitrificans.