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Thiosulfate-Oxidizing Medium

[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

K2HPO4......................................................................................... 2.0g

MgSO4·7H2O................................................................................ 0.1g

CaCl2·2H2O .................................................................................. 0.1g

FeCl3·6H2O................................................................................. 0.02g

(NH4)2SO4 solution................................................................100.0mL

Thiosulfate solution ...............................................................100.0mL

pH 7.8 ± 0.2 at 25°C

(NH4)2SO4 Solution:

Composition per 100.0mL:

(NH4)2SO4 .................................................................................... 0.1g

Preparation of (NH4)2SO4 Solution: Add the (NH4)2SO4 to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Thiosulfate Solution:

Composition per 100.0mL:

Na2S2O3·5H2O ............................................................................ 10.0g

Preparation of Thiosulfate Solution: Add the Na2S2O3·5H2O to

distilled/deionized water and bring volume to 100.0mL. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Preparation of Medium: Add components, except (NH4)2SO4 so

lution and thiosulfate solution, to distilled/deionized water and bring

volume to 800.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add the sterile

(NH4)2SO4 solution and the sterile thiosulfate solution. Mix thorough

ly. Adjust the pH to 7.8 if necessary. Aseptically distribute into sterile

tubes or flasks.

Use: For the isolation and cultivation of iron and sulfur bacteria.