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Thiothrix Agar

[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]

Thiothrix Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1003.0mL:

Agar ............................................................................................ 12.0g

NH4Cl ........................................................................................... 0.2g

Sodium acetate.............................................................................. 0.1g

K2HPO4....................................................................................... 0.01g

MgSO4·7H2O .............................................................................. 0.01g

CaSO4 (saturated solution).......................................................20.0mL

Na2S·9H2O solution ...................................................................3.0mL

Trace elements solution .............................................................5.0mL

pH 7.5 ± 0.2 at 25°C

Na2S·9H2O Solution:

Composition per 10.0mL:

Na2S·9H2O.................................................................................... 1.0g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C.

Trace Elements Solution:

Composition per liter:

FeSO4·7H2O.................................................................................. 0.7g

EDTA............................................................................................ 0.2g

ZnSO4·7H2O ............................................................................... 0.01g

MnSO4·4H2O............................................................................ 0.002g

H3BO3 ...................................................................................... 10.0mg

CO(NO3)2 .................................................................................. 1.0mg

Na2MoO4·2H2O ........................................................................ 1.0mg

CuSO4·5H2O...............................................................................5.0μg

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except Na2S·9H2O so

lution, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°–55°C. Aseptically add 3.0mL of sterile

Na2S·9H2O solution. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the cultivation and maintenance of Thiothrix nivea.