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Thiothrix Medium (DSMZ Medium 573)

[所属分类:培养基配方] [发布时间:2021-9-15] [发布人:网站管理员2] [阅读次数:] [返回]

Thiothrix Medium

(DSMZ Medium 573)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NH4Cl ........................................................................................... 0.2g

Na-acetate ..................................................................................... 0.1g

K2HPO4....................................................................................... 0.01g

MgSO4·7H2O.............................................................................. 0.01g

CaSO4 (saturated solution)......................................................20.0mL

Na2S·9H2O solution.................................................................10.0mL

Trace elements solution .............................................................5.0mL

pH 7.5 ± 0.2 at 25°C

Na2S·9H2O Solution:

Composition per 10.0mL:

Na2S·9H2O.................................................................................... 0.3g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis

tilled/deionized water and bring volume to 10.0mL. Sparge with N2.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Store an

aerobically.

Trace Elements Solution:

Composition per liter:

FeSO4·7H2O.................................................................................. 0.7g

EDTA............................................................................................ 0.2g

ZnSO4·7H2O ............................................................................ 10.0mg

H3BO3 ...................................................................................... 10.0mg

MnSO4·4H2O............................................................................. 2.0mg

Co(NO3)2.................................................................................. 1.0mg

Na2MoO4·4H2O ......................................................................... 1.0mg

CuSO4·5H2O...............................................................................5.0μg

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except Na2S·9H2O so

lution, to distilled/deionized water and bring volume to 990.0mL. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptical

ly add 10.0mL sterile Na2S·9H2O solution. Mix thoroughly. Aseptical

ly distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiothrix nivea.