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培养基配方
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Tissue Culture Medium NCTC 109 (TC Medium NCTC 109)
[所属分类:培养基配方] [发布时间:2021-9-16] [发布人:网站管理员2] [阅读次数:] [返回]
Tissue Culture Medium NCTC 109
(TC Medium NCTC 109)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per 1050.0mL:
NaCl .............................................................................................. 6.8g
NaHCO3 ........................................................................................ 2.2g
Glucose ......................................................................................... 1.0g
KCl................................................................................................ 0.4g
L-Cysteine.................................................................................... 0.26g
CaCl2·2H2O................................................................................... 0.2g
NaH2PO4 ..................................................................................... 0.14g
L-Glutamine................................................................................. 0.14g
MgSO4·7H2O ................................................................................ 0.1g
Sodium acetate............................................................................ 0.05g
Ascorbic acid .............................................................................. 0.05g
L-Alanine..................................................................................... 0.03g
L-Lysine....................................................................................... 0.03g
L-Arginine ................................................................................. 0.026g
L-Valine ..................................................................................... 0.025g
L-Leucine..................................................................................... 0.02g
Phenol Red.................................................................................. 0.02g
L-Histidine................................................................................. 0.019g
L-Threonine ............................................................................... 0.019g
L-Isoleucine ............................................................................... 0.018g
L-Tryptophan............................................................................ 0.017.g
L-Phenylalanine......................................................................... 0.017g
L-Tyrosine.................................................................................. 0.016g
Glycine...................................................................................... 0.014g
Tween™ 80............................................................................... 0.012g
L-Serine ..................................................................................... 0.011g
L-Cystine ..................................................................................... 0.01g
Glutathione.................................................................................. 0.01g
Cyanocobalamin ......................................................................... 0.01g
Deoxycytidine............................................................................. 0.01g
Deoxyguanosine.......................................................................... 0.01g
Deoxyadenosine.......................................................................... 0.01g
Thymidine................................................................................... 0.01g
L-Aspartic acid .........................................................................9.91mg
L-Glutamic acid........................................................................8.26mg
L-Arginine ................................................................................8.09mg
L-Ornithine ...............................................................................7.38mg
Nicotinamide adenine dinucleotide ...........................................7.0mg
L-Proline...................................................................................6.13mg
L-α-N-butyric acid ...................................................................5.51mg
L-Methionine ............................................................................4.44mg
L-Taurine ..................................................................................4.18mg
L-Hydroxyproline.....................................................................4.09mg
D-Glucosamine ...........................................................................3.2mg
Coenzyme A ..............................................................................2.5mg
Glucuronolactone.......................................................................1.8mg
Sodium glucuronate ...................................................................1.8mg
Choline chloride.......................................................................1.25mg
Cocarboxylase............................................................................1.0mg
Flavin adenine dinucleotide .......................................................1.0mg
Uridine triphosphate ..................................................................1.0mg
Nicotinamide adenine
dinucleotide phosphate ........................................................1.0mg
Vitamin A.................................................................................0.25mg
Calciferol .................................................................................0.25mg
i-Inositol.................................................................................0.125mg
p-Aminobenzoic acid.............................................................0.125mg
5-Methylcytosine .......................................................................0.1mg
Pyridoxine·HCl ....................................................................0.0625mg
Pyridoxal·HCl ...................................................................... 0.0625mg
Niacin................................................................................... 0.0625mg
Niacinamide......................................................................... 0.0625mg
Biotin ..................................................................................... 0.025mg
Folic acid ............................................................................... 0.025mg
Menadione ............................................................................. 0.025mg
Pantothenate........................................................................... 0.025mg
Riboflavin .............................................................................. 0.025mg
Thiamine·HCl ........................................................................ 0.025mg
α-Tocopherol phosphate ........................................................ 0.025mg
Serum............................................................................50.0–100.0mL
pH 7.2–7.4 at 25°C
Preparation of Medium: Add components, except serum, to dis
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad
just pH to 7.2–7.4 with 10% Na2CO3 solution. Filter sterilize. Asepti
cally add 50.0–100.0mL of sterile serum. Human serum, bovine serum,
horse serum, or fetal calf serum may be used. Mix thoroughly.
Use: For the cultivation of a wide variety of cell lines in tissue culture.