编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

Tissue Culture Medium Ham F10 (TC Medium Ham F10)

[所属分类:培养基配方] [发布时间:2021-9-16] [发布人:网站管理员2] [阅读次数:] [返回]

Tissue Culture Medium Ham F10

(TC Medium Ham F10)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1050.0mL:

NaCl.............................................................................................. 7.4g

Glucose ......................................................................................... 1.1g

Na2HPO4..................................................................................... 0.29g

KCl............................................................................................ 0.285g

L-Arginine ................................................................................. 0.211g

MgSO4·7H2O............................................................................ 0.153g

L-Glutamine ............................................................................ 0.1462g

Sodium pyruvate......................................................................... 0.11g

KH2PO4..................................................................................... 0.083g

CaCl2·2H2O .............................................................................. 0.044g

L-Cystine ................................................................................. 0.0315g

L-Lysine................................................................................... 0.0293g

L-Histidine................................................................................. 0.021g

L-Asparagine ............................................................................. 0.015g

L-Glutamic acid....................................................................... 0.0147g

L-Aspartic acid ........................................................................ 0.0133g

L-Leucine ................................................................................ 0.0131g

L-Proline.................................................................................. 0.0115g

L-Serine ................................................................................... 0.0105g

L-Alanine.................................................................................. 8.91mg

Glycine..................................................................................... 7.51mg

L-Phenylalanine........................................................................ 4.96mg

L-Methionine............................................................................ 4.48mg

Hypoxanthine............................................................................. 4.0mg

L-Threonine.............................................................................. 3.57mg

L-Valine ...................................................................................... 3.5mg

L-Isoleucine................................................................................ 2.6mg

L-Tyrosine ................................................................................ 1.81mg

Cyanocobalamin ........................................................................ 1.3mg

Folic acid ................................................................................... 1.3mg

Phenol Red................................................................................. 1.2mg

Thiamine·HCl ............................................................................ 1.0mg

FeSO4·7H2O............................................................................. 0.83mg

Calcium pantothenate ................................................................ 0.7mg

Thymidine.................................................................................. 0.7mg

Choline chloride....................................................................... 0.69mg

Niacinamide............................................................................... 0.6mg

L-Tryptophan.............................................................................. 0.6mg

i-Inositol................................................................................... 0.54mg

Riboflavin ................................................................................ 0.37mg

Lipoic acid ................................................................................. 0.2mg

Pyridoxine·HCl .......................................................................... 0.2mg

ZnSO4·7H2O .......................................................................... 0.028mg

Biotin ..................................................................................... 0.024mg

CuSO4·5H2O...............................................................................2.5μg

Fetal calf serum.............................................................50.0–100.0mL

pH 7.2–7.4 at 25°C

Preparation of Medium: Add components, except fetal calf serum,

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Adjust pH to 7.2–7.4 with 10% Na2CO3 solution. Filter sterilize. Asep

tically add 50.0–100.0mL of sterile fetal calf serum. Mix thoroughly.

Use: For the cultivation of a wide variety of cell lines in tissue culture.

在线客服

Tissue Culture Medium Ham F10 (TC Medium Ham F10)