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Tissue Culture Medium Eagle, HeLa (TC Medium Eagle, HeLa)

[所属分类:培养基配方] [发布时间:2021-9-16] [发布人:网站管理员2] [阅读次数:] [返回]

Tissue Culture Medium Eagle, HeLa

(TC Medium Eagle, HeLa)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1056.0mL:

NaCl ............................................................................................ 5.85g

NaHCO3 ...................................................................................... 1.68g

Glucose ......................................................................................... 0.9g

KCl............................................................................................ 0.373g

NaH2PO4 ................................................................................... 0.138g

MgCl2·6H2O................................................................................ 0.12g

CaCl2·2H2O................................................................................. 0.11g

L-Lysine................................................................................... 0.0269g

L-Isoleucine ............................................................................. 0.0262g

L-Leucine................................................................................. 0.0262g

L-Threonine ............................................................................. 0.0238g

L-Valine ................................................................................... 0.0234g

L-Tyrosine................................................................................ 0.0181g

L-Arginine ............................................................................... 0.0174g

L-Phenylalanine....................................................................... 0.0165g

L-Cystine ................................................................................... 0.012g

L-Histidine..................................................................................7.8mg

L-Methionine ..............................................................................7.5mg

Phenol Red.................................................................................5.0mg

L-Tryptophan ..............................................................................4.1mg

Folic acid..................................................................................0.44mg

Thiamine·HCl ..........................................................................0.34mg

Biotin .......................................................................................0.24mg

Pantothenic acid.......................................................................0.22mg

Pyridoxal·HCl ............................................................................0.2mg

Choline chloride.......................................................................0.14mg

Nicotinamide............................................................................0.12mg

Riboflavin ................................................................................0.04mg

Serum ......................................................................50.0mL–100.0mL

Glutamine solution.....................................................................6.0mL

pH 7.2–7.4 at 25°C

Glutamine Solution:

Composition per 100.0mL:

L-Glutamine................................................................................... 5.0g

NaCl (0.85% solution) ...........................................................100.0mL

Preparation of Glutamine Solution: Add the glutamine to the

0.85% NaCl solution. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glutamine and

serum, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Adjust pH to 7.2–7.4. Filter sterilize. Aseptically add 6.0mL of

sterile glutamine solution and 50.0–100.0mL of sterile serum. Human

serum, bovine serum, horse serum, or fetal calf serum may be used. Mix

thoroughly.

Use: For the cultivation and maintenance of HeLa and other cell lines

in tissue culture, and for studying the cytopathogenicity of viral agents.

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Tissue Culture Medium Eagle, HeLa (TC Medium Eagle, HeLa)