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Toluidine Blue DNA Agar

[所属分类:培养基配方] [发布时间:2021-9-17] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 10.0g

NaCl............................................................................................ 10.0g

Tris(hydroxymethyl)aminomethane buffer................................... 6.1g

Deoxyribonucleic acid.................................................................. 0.3g

Toluidine Blue O....................................................................... 0.083g

CaCl2, anhydrous....................................................................... 1.1mg

pH 9.0 ± 0.2 at 25°C

Preparation of Medium: Add tris(hydroxymethyl)aminomethane

buffer to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Adjust pH to 9.0. Add the remaining components, except Tol

uidine Blue O. Mix thoroughly. Gently heat and bring to boiling. Add

Toluidine Blue O. Mix thoroughly. If used the same day, sterilization is

not necessary. Cool to 50°C. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the cultivation and differentiation of Staphylococcus aureus

from foods.