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Toluidine Blue DNA Agar

[所属分类:培养基配方] [发布时间:2021-9-17] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 10.0g

NaCl............................................................................................ 10.0g

Tris(hydroxymethyl)aminomethane buffer................................... 6.1g

Deoxyribonucleic acid (DNA)...................................................... 0.3g

Toluidine Blue O....................................................................... 0.083g

CaCl2, anhydrous....................................................................... 1.1mg

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except Toluidine Blue

O, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Gently heat and bring to boiling. Add Toluidine Blue O. Mix

thoroughly. Medium does not have to be sterilized if used immediately.

Pour into sterile Petri dishes or distribute into sterile tubes. Allow tubes

to cool in a slanted position.

Use: For the cultivation and differentiation of bacteria based on their

production of deoxyribonuclease (DNase). Bacteria that produce DNase

turn the medium pink.