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Tomato Dextrin Yeast Medium

[所属分类:培养基配方] [发布时间:2021-9-17] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tomato paste............................................................................... 20.0g

Dextrin ........................................................................................ 20.0g

Agar ............................................................................................ 20.0g

Baker’s yeast............................................................................... 10.0g

CoCl2·6H2O ............................................................................... 5.0mg

pH 7.2–7.4 at 25°C

Preparation of Medium: Add components, except agar, to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad

just pH to 7.2–7.4. Add agar. Gently heat and bring to boiling. Distrib

ute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Streptomyces avermitilis.