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Treponema Medium

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

Treponema Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Heart infusion broth, modified...............................................450.0mL

Spirolate broth........................................................................450.0mL

Rabbit serum, inactivated ......................................................100.0mL

pH 7.4 ± 0.2 at 25°C

Heart Infusion Broth, Modified:

Composition per liter:

Beef heart, solids from infusion................................................ 500.0g

Tryptose ...................................................................................... 10.0g

NaCl .............................................................................................. 5.0g

Asparagine .................................................................................... 2.5g

Sodium thioglycolate.................................................................... 2.5g

Pancreatic digest of casein............................................................ 2.5g

Preparation of Heart Infusion Broth, Modified: Add compo

nents to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 25°C.

Spirolate Broth:

Composition per liter:

Pancreatic digest of casein.......................................................... 15.0g

Glucose ......................................................................................... 5.0g

Yeast extract.................................................................................. 5.0g

NaCl.............................................................................................. 2.5g

L-Cysteine·HCl·H2O...................................................................... 1.0g

Sodium thioglycolate.................................................................... 0.5g

Palmitic acid ............................................................................... 0.05g

Stearic acid.................................................................................. 0.05g

Oleic acid.................................................................................... 0.05g

Linoleic acid ............................................................................... 0.05g

Preparation of Spirolate Broth: Add components to distilled/de

ionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 25°C.

Preparation of Medium: Aseptically combine 450.0mL of cooled,

sterile spirolate broth, 450.0mL of cooled, sterile heart infusion broth,

modified, and 100.0mL of rabbit serum. Mix thoroughly. Aseptically

distribute into sterile tubes or flasks.

Use: For the cultivation of treponemes.