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Treponema Medium

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

Treponema Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 500.0mL:

Beef heart, solids from infusion................................................ 250.0g

Sucrose........................................................................................ 50.0g

Tryptose ........................................................................................ 5.0g

NaCl.............................................................................................. 2.5g

Yeast extract.................................................................................. 2.5g

Agar .............................................................................................. 0.5g

Sodium thioglycolate.................................................................. 0.38g

MgSO4 ........................................................................................ 0.05g

Horse serum, inactivated .......................................................100.0mL

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse serum, to

distilled/deionized water and bring volume to 400.0mL. Mix thorough

ly. Adjust pH to 7.4. Gently heat and bring to boiling. Distribute into

tubes in 4.0mL volumes. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 25°C. Prior to inoculation, add 1.0mL sterile horse se

rum to each tube.

Use: For the cultivation and maintenance of Treponema pallidum and

other Treponema species.