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Treponema Medium 1

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

Treponema Medium 1

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Thioglycolate agar USP, alternate..........................................900.0mL

Normal calf serum .................................................................100.0mL

pH 7.1 ± 0.2 at 25°C

Thioglycolate Agar USP, Alternate:

Composition per 900.0mL:

Pancreatic digest of casein.......................................................... 15.0g

Ionagar No. 2 ................................................................................ 7.0g

Glucose ......................................................................................... 5.5g

Yeast extract.................................................................................. 5.0g

NaCl .............................................................................................. 2.5g

L-Cystine ....................................................................................... 0.5g

Sodium thioglycolate .................................................................... 0.5g

Preparation of Thioglycolate Agar USP, Alternate: Add com

ponents to distilled/deionized water and bring volume to 900.0mL.

Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C.

Preparation of Medium: Aseptically combine 900.0mL of cooled

sterile thioglycolate agar USP, alternate, and 100.0mL of calf serum.

Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the cultivation of treponemes.