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Treponema Medium 2

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

Treponema Medium 2

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 30.0g

Ionagar No. 2 ................................................................................ 7.0g

Glucose ......................................................................................... 5.0g

Yeast extract.................................................................................. 5.0g

NaCl .............................................................................................. 2.5g

L-Cysteine·HCl·H2O...................................................................... 2.0g

Rabbit serum ..........................................................................100.0mL

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except rabbit serum,

to distilled/deionized water and bring volume to 900.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile

rabbit serum. Mix thoroughly. Pour into sterile Petri dishes or dis

tribute into sterile tubes.

Use: For the cultivation of treponemes.