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Treponema Medium 3

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

Treponema Medium 3

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Spirolate agar .........................................................................675.0mL

Brain heart infusion broth ......................................................225.0mL

Rabbit serum ..........................................................................100.0mL

Spirolate Agar:

Composition per liter:

Pancreatic digest of casein.......................................................... 15.0g

Ionagar No. 2 ................................................................................ 7.0g

Glucose ......................................................................................... 5.0g

Yeast extract.................................................................................. 5.0g

NaCl .............................................................................................. 2.5g

L-Cysteine·HCl·H2O...................................................................... 1.0g

Sodium thioglycolate .................................................................... 0.5g

Palmitic acid ............................................................................... 0.05g

Stearic acid.................................................................................. 0.05g

Oleic acid .................................................................................... 0.05g

Linoleic acid ............................................................................... 0.05g

Preparation of Spirolate Agar: Add components to distilled/de

ionized water and bring volume to 1.0L. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 25°C.

Brain Heart Infusion Broth:

Composition per liter:

Pancreatic digest of gelatin ......................................................... 14.5g

Brain heart, solids from infusion .................................................. 6.0g

Peptic digest of animal tissue ....................................................... 6.0g

NaCl.............................................................................................. 5.0g

Casein ........................................................................................... 5.0g

Glucose ......................................................................................... 3.0g

Na2HPO4....................................................................................... 2.5g

Preparation of Brain Heart Infusion Broth: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 25°C.

Preparation of Medium: Aseptically combine 675.0mL of cooled,

sterile spirolate broth, 225.0mL of cooled, sterile brain heart infusion

broth, and 100.0mL of rabbit serum. Mix thoroughly.

Use: For the cultivation of treponemes.