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Treponema Medium, Prereduced

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar .............................................................................................. 1.6g

Glucose ......................................................................................... 1.4g

Cysteine·HCl·H2O ...................................................................... 0.64g

(NH4)2SO4 .................................................................................... 0.5g

Polypeptone™ .............................................................................. 0.5g

Starch, soluble............................................................................... 0.5g

Yeast extract.................................................................................. 0.5g

Resazurin ................................................................................... 1.6mg

Salts solution..........................................................................500.0mL

Bovine rumen fluid................................................................280.0mL

pH 7.2–7.5 at 25°C

Salts Solution:

Composition per liter:

NaHCO3...................................................................................... 10.0g

NaCl.............................................................................................. 2.0g

K2HPO4......................................................................................... 1.0g

KH2PO4......................................................................................... 1.0g

CaCl2............................................................................................. 0.2g

MgSO4 .......................................................................................... 0.2g

CoCl........................................................................................... 3.4mg

MnSO4 ....................................................................................... 3.4mg

NaMoO4..................................................................................... 3.4mg

Preparation of Salts Solution: Add components to distilled/deion

ized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except bovine rumen

fluid, to distilled/deionized water and bring volume to 720.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 280.0mL of

sterile bovine rumen fluid. Mix thoroughly. Aseptically and anaerobi

cally distribute into sterile tubes or flasks under 100% N2.

Use: For the cultivation of fecal and intestinal treponemes.