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Tributyrin Agar

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

Tributyrin Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Tributyrin (glyceryl tributyrate).................................................. 10.0g

Peptone ......................................................................................... 5.0g

Yeast extract.................................................................................. 3.0g

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a prepared medium from Oxoid

Unipath.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes.

Use: For the cultivation and enumeration of lipolytic fungi and bacte

ria, especially Staphylococcus species, Flavobacterium species, Clos

tridium species, and Pseudomonas species from butter. Lipolytic bac

teria appear as colonies surrounded by a clear zone.