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Tributyrin HiVeg Agar Base with Tributyrin

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Plant peptone................................................................................. 5.0g

Yeast extract.................................................................................. 3.0g

Tributyrin (glyceryl tributyrate)...............................................10.0mL

pH 7.5 ± 0.2 at 25°C

Source: This medium, without tributyrin, is available as a premixed

powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 990.0mL. Add 10.0mL of tributyrin. Mix

thoroughly. Gently heat and bring to boiling. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–

50°C. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the detection of lipolytic microorganisms.