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Trichococcus Medium

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

Trichococcus Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 10.0g

Na2SO4.......................................................................................... 4.0g

MgCl2·6H2O.................................................................................. 1.1g

KCl................................................................................................ 0.7g

Glucose solution ......................................................................30.0mL

pH 7.3 ± 0.2 at 25°C

Glucose Solution:

Composition per 30.0mL:

Glucose ......................................................................................... 3.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 30.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glucose solu

tion, to distilled/deionized water and bring volume to 970.0mL. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptical

ly add 30.0mL of sterile glucose solution. Mix thoroughly. Aseptically

distribute into sterile tubes or flasks.

Use: For the cultivation of Trichococcus flocculiformis.