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Trichomonas HiVeg Agar Base with Serum
[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]
Trichomonas HiVeg Agar Base with Serum
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Plant extract No. 2 ...................................................................... 25.0g
NaCl .............................................................................................. 6.5g
Glucose ......................................................................................... 5.0g
Agar .............................................................................................. 1.0g
Horse serum.............................................................................80.0mL
pH 6.4 ± 0.2 at 25°C
Source: This medium, without horse serum, is available as a pre
mixed powder from HiMedia.
Horse Serum:
Composition per 80.0mL:
Horse serum.............................................................................80.0mL
Preparation of Horse Serum: Gently heat sterile horse serum to
56°C for 30 min. Aseptically adjust pH to 6.0 with 0.1N HCl. Use
immediately.
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 920.0mL. Mix thorough
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 80.0mL of freshly
prepared sterile horse serum. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation of Trichomonas vaginalis.