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Trichomonas Medium No. 2

[所属分类:培养基配方] [发布时间:2021-9-22] [发布人:网站管理员2] [阅读次数:] [返回]
Trichomonas Medium No. 2

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 22.5g
Liver digest ................................................................................. 18.0g
Pancreatic digest of casein.......................................................... 17.0g
NaCl .............................................................................................. 5.0g
Pancreatic digest of soybean meal ................................................ 3.0g
K2HPO4......................................................................................... 2.5g
Chloramphenicol....................................................................... 0.125g
Horse serum ...........................................................................250.0mL
Calcium pantothenate (0.5% solution).......................................1.0mL
pH 6.2 ± 0.2 at 25°C
Source: This medium is available as a prepared medium from Oxoid Un
ipath.
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 750.0mL. Mix thorough
ly. Autoclave for 15 min at 5 psi pressure–108°C. Cool to 45°–50°C.
Aseptically add 250.0mL of sterile horse serum. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the isolation of Trichomonas vaginalis.
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