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Trichomonas Selective Medium
[所属分类:培养基配方] [发布时间:2021-9-22] [发布人:网站管理员2] [阅读次数:] [返回]
Trichomonas Selective Medium
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Liver digest ................................................................................. 25.0g
NaCl .............................................................................................. 6.5g
Glucose ......................................................................................... 5.0g
Agar .............................................................................................. 1.0g
Horse serum .............................................................................80.0mL
Antibiotic inhibitor ..................................................................10.0mL
pH 6.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
Horse Serum:
Composition per 80.0mL:
Horse serum.............................................................................80.0mL
Preparation of Horse Serum: Gently heat sterile horse serum to
56°C for 30 min. Aseptically adjust pH to 6.0 with 0.1N HCl. Use im
mediately.
Antibiotic Inhibitor:
Composition per 10.0mL:
Streptomycin.......................................................................... 500.0mg
Penicillin G ....................................................................... 1,000,000U
Preparation of Antibiotic Inhibitor: Add components to dis
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except horse serum
and antibiotic inhibitor, to distilled/deionized water and bring volume
to 910.0mL. Mix thoroughly. Gently heat and bring to boiling. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti
cally add 80.0mL of freshly prepared sterile horse serum and 10.0mL
of sterile antibiotic inhibitor. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation of Trichomonas vaginalis from specimens
with a mixed bacterial flora.