培养基配方

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Trichomonas Selective Medium

[所属分类:培养基配方] [发布时间:2021-9-22] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Liver digest ................................................................................. 25.0g

NaCl .............................................................................................. 6.5g

Glucose ......................................................................................... 5.0g

Agar .............................................................................................. 1.0g

Horse serum .............................................................................80.0mL

Antibiotic inhibitor ..................................................................10.0mL

pH 6.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath.

Horse Serum:

Composition per 80.0mL:

Horse serum.............................................................................80.0mL

Preparation of Horse Serum: Gently heat sterile horse serum to

56°C for 30 min. Aseptically adjust pH to 6.0 with 0.1N HCl. Use im

mediately.

Antibiotic Inhibitor:

Composition per 10.0mL:

Streptomycin.......................................................................... 500.0mg

Penicillin G ....................................................................... 1,000,000U

Preparation of Antibiotic Inhibitor: Add components to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except horse serum

and antibiotic inhibitor, to distilled/deionized water and bring volume

to 910.0mL. Mix thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti

cally add 80.0mL of freshly prepared sterile horse serum and 10.0mL

of sterile antibiotic inhibitor. Mix thoroughly. Aseptically distribute

into sterile tubes or flasks.

Use: For the cultivation of Trichomonas vaginalis from specimens

with a mixed bacterial flora.