培养基配方

您现在的位置：网站首页 >> 培养基配方

Trichosel™ Broth, Modified

[所属分类:培养基配方] [发布时间:2021-9-22] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 12.0g

Yeast extract.................................................................................. 5.0g

Liver extract.................................................................................. 2.0g

Maltose ......................................................................................... 2.0g

L-Cysteine·HCl.............................................................................. 1.0g

Agar .............................................................................................. 1.0g

Chloramphenicol........................................................................... 0.1g

Methylene Blue.......................................................................... 3.0mg

Horse serum.............................................................................50.0mL

pH 6.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components, except horse serum, to

distilled/deionized water and bring volume to 950.0mL. Mix thorough

ly. Gently heat while stirring and bring to boiling. Autoclave for 15 min

at 13 psi pressure–118°C. Cool to 45°–50°C. Aseptically add 50.0mL

of sterile horse serum. Mix thoroughly. Aseptically distribute into ster

ile tubes or flasks.

Use: For the isolation and cultivation of Trichomonas species.