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Trimethylamine N-Oxide Medium (TMAO Medium)

[所属分类:培养基配方] [发布时间:2021-9-22] [发布人:网站管理员2] [阅读次数:] [返回]

Trimethylamine N-Oxide Medium

(TMAO Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef extract................................................................................. 10.0g

Peptone ....................................................................................... 10.0g

NaCl.............................................................................................. 5.0g

Agar .............................................................................................. 2.0g

Trimethylamine N-oxide............................................................... 1.0g

Yeast extract.................................................................................. 1.0g

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and

bring to boiling. Distribute into screw-capped tubes in 4.0mL vol

umes. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes

to cool in an upright position.

Use: For the cultivation and differentiation of Campylobacter species

from foods. Campylobacter jejuni and Campylobacter coli will not

grow.