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Trypaflavin Nalidixic Acid Serum Agar (TNSA Agar)

[所属分类:培养基配方] [发布时间:2021-9-22] [发布人:网站管理员2] [阅读次数:] [返回]

Trypaflavin Nalidixic Acid Serum Agar

(TNSA Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Ionagar No. 2 .............................................................................. 12.0g

Peptone........................................................................................ 10.0g

Beef extract ................................................................................... 3.0g

H2O ........................................................................................926.5mL

Bovine serum, heat inactivated................................................50.0mL

Nalidixic acid solution .............................................................20.0mL

Trypaflavin solution...................................................................3.5mL

pH 7.2–7.4 at 25°C

Nalidixic Acid Solution:

Composition per 10.0mL:

Nalidixic acid.............................................................................. 0.02g

Preparation of Nalidixic Acid Solution: Add nalidixic acid to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Filter sterilize.

Trypaflavin Solution:

Composition per 10.0mL:

Trypaflavin.................................................................................... 0.1g

Preparation of Trypaflavin Solution: Add trypaflavin to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components—except bovine serum,

nalidixic acid solution, and trypaflavin solution—to distilled/deionized

water and bring volume to 926.5mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C. Aseptically add 50.0mL of sterile bovine serum, 20.0mL

of sterile nalidixic acid solution, and 3.5mL of trypaflavin solution.

Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the isolation and cultivation of Listeria species from preen

riched specimens.