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Trypanosome Medium

[所属分类:培养基配方] [发布时间:2021-9-22] [发布人:网站管理员2] [阅读次数:] [返回]

Trypanosome Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1300.0mL:

Solid phase....................................................................................1.0L

Liquid phase (Locke’s solution) ............................................300.0mL

pH 7.2–7.4 at 25°C

Solid Phase:

Composition per liter:

Agar ............................................................................................ 15.0g

NaCl.............................................................................................. 8.0g

Peptone ......................................................................................... 5.0g

Beef extract................................................................................... 3.0g

Rabbit blood, defibrinated .....................................................300.0mL

Preparation of Solid Phase: Add components, except rabbit

blood, to distilled/deionized water and bring volume to 700.0mL. Mix

thoroughly. Adjust pH to 7.2–7.4. Gently heat and bring to boiling. Au

toclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Asep

tically add 300.0mL of sterile defibrinated rabbit blood. Mix

thoroughly. Distribute 10.0mL aliquots into sterile screw-capped

tubes. Allow to solidify in a slanted position.

Liquid Phase (Locke’s Solution):

Composition per liter:

NaCl.............................................................................................. 8.0g

Glucose ......................................................................................... 2.5g

KH2PO4......................................................................................... 0.3g

CaCl2............................................................................................. 0.2g

KCl................................................................................................ 0.2g

Preparation of Liquid Phase (Locke’s Solution): Add compo

nents to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically overlay agar slants (solid

phase) with 3.0mL per tube of sterile liquid phase (Locke’s solution).

Use: For the cultivation of Leishmania donovani, Leishmania brazil

iensis, Trypanosoma gambiense, and Trypanosoma rhodesiense.