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Trypticase™ Peptone Glucose Yeast Extract Broth with Trypsin (TPGYT Broth)

[所属分类:培养基配方] [发布时间:2021-9-23] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase™ Peptone Glucose

Yeast Extract Broth with Trypsin

(TPGYT Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1067.0mL:

Pancreatic digest of casein.......................................................... 50.0g

Yeast extract................................................................................ 20.0g

Peptone.......................................................................................... 5.0g

Glucose ......................................................................................... 4.0g

Sodium thioglycolate .................................................................... 1.0g

Trypsin solution .......................................................................67.0mL

pH 7.0 ± 0.2 at 25°C

Trypsin Solution:

Composition per 100.0mL:

Trypsin .......................................................................................... 1.5g

Preparation of Trypsin Solution: Add trypsin to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, except trypsin solu

tion, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Gently heat and bring to boiling. Distribute into tubes in

15.0mL volumes. Autoclave for 10 min at 15 psi pressure–121°C. Im

mediately prior to use, aseptically add 1.0mL of sterile trypsin solution

to each tube. Mix thoroughly.

Use: For the cultivation of Clostridium botulinum.